Multicenter comparison of molecular methods for detection of Legionella spp. in sputum samples.
نویسندگان
چکیده
Legionellosis can be diagnosed by PCR using sputum samples. In this report, the methods of nine laboratories for 12 sputum samples with Legionella pneumophila and Legionella longbeachae are compared. We conclude that (i) liquefaction prevents PCR inhibition, (ii) the employed mip gene PCRs detected L. pneumophila only, and (iii) the 16S rRNA gene PCR detected both Legionella species and is preferred for the diagnosis of legionellosis.
منابع مشابه
Isolation and identification of Legionella spp. from different aquatic sources in south-west of Iran by molecular &culture methods
Legionella pneumophila, the causative agent of Legionnaires’ diseases (LD) is usually transmitted to humans via inhalation of aerosols from contaminated natural and manmade water sources. These organisms may become fatal especially in immunocompromised patients and LD is the one of the important disease from a public health perspective. This survey investigated the frequency of Legionella spp. ...
متن کاملمقایسه حساسیت پرایمرهای مختلف اختصاصی ناحیه 16srDNAدر شناسایی گونه‌های باکتری لژیونلا در نمونه‌های آبی
Background and Objectives: Legionella are gram-negative bacteria widely dispersed in natural and man-made water sources. Some Legionella species are pathogenic and could cause respiratory infections. Cultivation technique is the conventional method for the detection of Legionella spp. in aquatic samples. However, the method has low sensitivity and require prolonged incubation period. Theref...
متن کاملشناسایی لژیونلا پنوموفیلا از نمونه‌های خلط بیماران مبتلا به پنومونی با روش کشت، ایمنوفلورسانس مستقیم و PCR
Abstract Background: Community acquired pneumonia (CAP) is the main cause of mortality and morbidity world wide. Legionella pneumophila is identified as the fourth agent that causes CAP. The aim of this study was to define the prevalence of L. pneumophila among hospitalized children by culture, direct immune-fluorescence, (DFA) and PCR. Methods: In this study 210 sputum samples were collect...
متن کاملبررسی مقایسه‌ای تشخیص لژیونلا پنوموفیلا به دو روش کشت خلط و آزمون آنتی ژن ادراری EIA در مبتلایان به عفونت تنفسی حاد
Background : Community acquired pneumonia (CAP) is a common health problem and one of the main mortality factors worldwide. Legionella pneumophila is one of the most common responsible microorganisms for CAP and may lead to severe complications if left untreated. The present study was conducted to determine the frequency of this organism in patients with CAP. Materials and methods : We enrolled...
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ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 45 10 شماره
صفحات -
تاریخ انتشار 2007